Application of lectin microarray to crude samples: differential glycan profiling of lec mutants.

نویسندگان

  • Youji Ebe
  • Atsushi Kuno
  • Noboru Uchiyama
  • Shiori Koseki-Kuno
  • Masao Yamada
  • Takashi Sato
  • Hisashi Narimatsu
  • Jun Hirabayashi
چکیده

We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lec mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.

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عنوان ژورنال:
  • Journal of biochemistry

دوره 139 3  شماره 

صفحات  -

تاریخ انتشار 2006